For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Bookings are free, but you will need to use iLab to book. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The dual monochromator optics allow the. Additionally, user-installable options allow the SpectraMax i3 System to. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. 1) Select the correct plate and the wells that you want to measure. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The guide also covers the software features, the specifications, and the accessories of the systems. Detection modalities include absorbance (UV-Vis Abs) and. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. A preconfigured protocol is available in the software to. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. 1. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Here, the CVs for each plate were less than 3, and the difference between mean. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. ATP standard curves read on SpectraMax microplate readers. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Fluorescence Microplate Reader Comparison Chart. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Products : General, Microplate Readers. Molecular Devices. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). SpectraMax Microplate Readers. SAN JOSE, CA — Molecular Devices, LLC. Assays vary depending on. Figure 3. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The instrument uses a paired monochromator and xenon flash. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 2 only: SpectraMax Mini Update. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. FI assay. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. , a. Microplate automation and detection instruments for walk away operation and high quality results. Molecular Devices SpectraMax M2 Multimode Plate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate reader settings. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Other features and benefits include: Eliminate changing out filters. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Time-. \ Figure 3. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Italic and boldface type are used for emphasis. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 304 signal level saturation. Add to Cart. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. 2. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 0 out of 5 $ 5,999. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. We would like to show you a description here but the site won’t allow us. Microplate Readers. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Learn more about its features, specifications, and applications in this user. Turn the reader off by pulling the power cord. . The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Avoid losing high signal. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. 0200-6263. For readers without a cuvette port, these options are not shown in the software. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Sale. edu) to be added as a trained user in iLab. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 997 (n = 3). Download PDF. Figure 1. Example: [Drawer]. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Align the two slots on the bottle holder with the two pegs on the rail. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 1 software Download page. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Offer Ends In: 2 Days 10: 40: 19. We would like to show you a description here but the site won’t allow us. Offer Ends In: 18 Days 19: 38: 21. Blank subtraction and standard curve fitting were done automatically by the software. SpectraMax Microplate Readers. Use the provided 3. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Measuring platelet aggregation with a microplate reader. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Maximum signal with lowest background. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 2 only) SpectraMax M-series Microplate readers. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. It can run samples based on pre-defined. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Use similar settings for other Molecular Devices readers with fluorescence detection mode. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. 300 µL of Detergent Compatible Bradford reagent was added to each well. Plate chamber design of the SpectraMax category 1 microplate readers. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Intended Use/Discipline:Biological. 470 nm). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Contact our support team with your questions regarding Molecular Devices products and applications. Download PDF. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. A: Microplate Preparation. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. In addition, the SpectraMax® iD5 can be expanded to include bottom. It also integrates with the. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Table of Contents. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Endpoint, kinetic, spectrum. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. 1. Cell Cytotoxicity assay procedure. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Type:Spectrophotometer. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Highly customizable low volume. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Temperature control. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. 76358-624. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The University of Rouen in Normandy uses the SpectraMax iD3 and. The powerful analysis tools in the software generate a standard curve and calculated data. 316 grating motor driver faulty. Other technologies such as imaging. 20 μL of Component A was added to each well of the microplate. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. 6% for the on-board optics (monochromators). Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 2. MPN:SpectraMax Plus. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. . microplate reader. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. flexibility of monochromator-based systems. M2. Sensitive fluorescent quantitation of DNA with the Quant-iT. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. flat meniscus), but is approx 15 mL. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The drawing shows a standard microplate of 15 mm height covered with a lid. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The plate reader optics are tunable for. The calculated LOQ values were 0. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. 0 out of 5 $ 11,999. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. 315 can't find zero order. Cuvette Microplate wells. For more sample throughput, both readersSupplier: Molecular Devices. Find regional support. We use Spectramax M5 microplate reader in our lab. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. . Fill a clean plate with water. The advanced optical and. Figure 1. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Microplate Readers. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. . Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 380 nm/Exc. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The chamber contains springs that position the cuvette in the proper alignment for a read. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax 190 reader can be integrated with Molecular . SAN JOSE, CA — Molecular Devices, LLC. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. SpectraMax i3x Multi-Mode Detection Platform. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. More Product Information. Example: [Drawer]. Or, with the reader specifications noted from the Instrument Description section of your reader's. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 020 fmol 0. SpectraMax Plus: SpectraMax Plus. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 030 fmol Fluorescence Polarization (FP). Find our technical support information along with contact details. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The price range of a reader starts at 2. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Instrument settings used with the SpectraMax M5 Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The screw remains inside the instrument. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Add to Cart. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 0 out of 5 $ 23,995 $ 22,495. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Molecular Interactions. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. limit of detection (LLD) for ATP was estimated for each microplate reader using the . SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The 2-channel configuration is available with 0, 2, or 4 injectors. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. B: Selection of Excitation Wavelength. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. A new technical approach to platelet aggregation studies. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. It was also read on the monochromator-based. Molecular Devices Spectramax iD3 Microplate Reader. Figure 1. Nucleic Acid and Protein Quantitation. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The plate reader optics are tunable for absorbance. Category 2 and 3 microplate readers use gradient heating as well. Step 2: Launch SoftMax Pro Software. Figure 2-23: Bottle holder To install the bottle holder: 1. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. . 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Compact designs. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. The dual monochromator optics allow. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Table 2 summarizes the results for the uniformity test. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. Microplate readers’ standard detection modes. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. 6% for the on-board optics (monochromators). The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Total volume (except deep. The SpectraMax 190 reader can be integrated with Molecular . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. 58% for the detection module and 2. microplate reader. The CellTiter-Glo assay from Promega uses luciferase. flexibility of monochromator-based systems. This pathlength correction is accomplished only with the use of. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. e. Brochure. These robust readers have been placed in labs from. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. Temperature control and shaking come as standard. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Detailed specifications of each model reader are reported in Section 7, “Specifications”. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Fully tested with validations, warranty. begin reading microplates with seven mouse clicks. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Path Check Pathlength Measurement every Gemini EM Reader. Multimode Microplate Reader. The dual monochromator optics allow the widest range of. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page.